Structural Analysis of Avian Encephalomyelitis Virus Polyprotein for Development of Multi Epitopes Vaccine Using Immunoinformatics Approach

Avian Encephalomyelitis (AE) is the disease caused by avian encephalomyelitis virus (AEV). The mainly affects young birds nervous system worldwide causing high morbidity and variable mortality rate in chicks noticed egg dropping hatchability mature hens. Vaccination only way to control AEV infection since there no treatment yet encephalomyelitis. This study aimed use immunoinformatics approaches predict multi epitopes vaccine from polyprotein that could elicit both B T cells. construct comprises 482 amino acids obtained predicted against cells IEDB server, adjuvant, linkers 6-His-tag. chimeric was potentially antigenic nonallergic demonstrated thermostability hydrophilicity protparam server. solubility of measured comparison E. coli proteins. stability also assessed disulfide bonds engineering reduce mobility regions designed vaccine. Furthermore molecular dynamics simulation further strengthen Tertiary structure after prediction, refinement used for docking with chicken alleles BF2*2101 BF2*0401 process favourable binding energy score -337.47 kcal/mol -326.87 kcal/mol, respectively. Molecular cloning potential clonability pET28a(+) vector. guarantee efficient translation expression within suitable